Fire Haired
03-15-2014, 10:32 AM
63 of these individuals are from Wilde 2014 (http://www.ancestraljourneys.org/bibliography.shtml#Wilde2014) and three are from Newton 2011 (http://www.ancestraljourneys.org/bibliography.shtml#Newton2011). I am making this thread so I and others have a source with this ancient DNA organized.
Map of these results. (https://mapsengine.google.com/map/edit?mid=zpAcd_2YNln8.kiAdXb0B2q5I) Many of the same rules apply, I suggest clicking on the name list on the left not on the map since many of them are on the exact same location so you can't view all of them.
How individuals info is setup: I put the name of the individual(EX: BEN3, SYM11, etc.) and in ()'s their Archaeological date(EX: EBA-Yam, EBA-Cat2, etc.) and location(found by plugging in latitude and longitude to this site (http://www.latlong.net/) and click here (https://mapsengine.google.com/map/edit?mid=zm0B1VqcS1ZE.kixFYqDlP5v8) for even more accurate locations). The first haplogroup listed is my prediction based on HVR1 mutations(aka differences to rCRS) given in Table.S1 of Wilde 2014 (http://www.ancestraljourneys.org/bibliography.shtml#Wilde2014). I used this predictor (http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&sqi=2&ved=0CC0QFjAA&url=http%3A%2F%2Fdna.jameslick.com%2F&ei=nB0kU-W2KoqsyAHKwIGoCw&usg=AFQjCNFGhJKVXY_TyAOJF2QQwvI2OBBd2A&sig2=75P8dZJIKZ90JZJQc4MSVQ&bvm=bv.62922401,d.aWc) and this mtDNA tree (http://www.phylotree.org/tree/main.htm)to make my predictions. The haplogroup in ()'s is what Wilde 2014 (http://www.ancestraljourneys.org/bibliography.shtml#Wilde2014) reported based on coding region mutations(aka differences to rCRS) in Table.S2, after that I give some reasoning why I predicted a certain haplogroup.
For every individual I put the pigmentation SNP's in this order rs12913832, rs16891982, rs1042602 because that is the order it is in in Table.S1 of Wilde 2014 (http://www.ancestraljourneys.org/bibliography.shtml#Wilde2014). rs12913832 (http://snpedia.com/index.php/Rs12913832) is associated with eye color, according to SNPedia A/A alleles is brown eye color 80% of the time, A/G is brown eye color, G/G is blue eye color 99% of the time. According to the 8-plex system (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3694299/)G/G can be any light color and A/G or A/A just mean not blue. rs16891982 (http://www.snpedia.com/index.php/Rs16891982)is associated with skin and hair color, according to SNPedia G/G alleles are associated with light skin and C/G or C/C in Europeans makes them 7x more likely to have black hair. G/G alleles are around 80% or more on average in Europeans and around 50% in near easterns(click here (http://mbe.oxfordjournals.org/content/24/3/710/F3.expansion.html)). According to Wikipedia rs1042602 (http://snpedia.com/index.php/Rs1042602) alleles G/G are associated with light skin and are about 40-50% in Europeans and near easterns(click here (http://mbe.oxfordjournals.org/content/24/3/710/F3.expansion.html)).
I split up the individuals according to their archeological date and within that their location. For sections EBA-Yam and EBA-Cat I copied the format, regions, and order of individuals from Maciamo at Eupedia.com(click here (http://www.eupedia.com/genetics/haplogroups_of_bronze_age_proto-indo-europeans.shtml)) and i also copied his format for the ENL section but not the regions and order of individuals. You can see that Maciamo's predictions based on HVR1 mutations are nearly identical to mine and Jean Manco made many of the same predictions at her site ancestral journeys(click here (http://www.ancestraljourneys.org/ancientdna.shtml)).
I strongly dis agree with some of Maciamo's predictions. Example: there are seven individuals in the archeological date EBA-Cat1 or EBA-Cat2 listed as having mtDNA haplogroup U(Table.S2) and with 16356C a their only HVR1 mutation. There are four U subclades with that mutation U5b1i, U2e3, U3a1c, and U4 and for every single one of those individuals Maciamo predicted U4. U4 makes the most sense but I think he needs to be more conservative. There are many other examples where I think Maciamo needed to be more conservative or more liberal.
For total mtDNA percentages I ordered them in numbers, 1. means it is not a subclade of any haplogroups listed, 2. its 1.'s subclade's 3. its 2.'s subclades and so on. The * means that its a independent percentage that isn't raised because its a subclade of a popular haplogroup without many subclades. In parenthesis I put the reported haplogroup according to coding region mutations.
EBA-Yam(5,000-4,500BP)
mtDNA=28
1.H(H)=7 25%* of total, sub=0
1.U(U, ?, U/K)=7 25%* of total, sub=7
2.U5(U, ?)=4 57%* of U(U, ?, U/K), 14.28%* of total, sub=3(U5(U)=1)
3.U5a1(U, ?)=3 100% of U5(U, ?), 57% of U(U, ?, U/K), 14.28% of total, sub=0(U5a1(?)=1, U5a1(U)=2)
2.U/K(U/K)=1 14.28%* of U(U, ?, U/K), 3.57%* of total, sub=0
2.U2e(U)=1 14.28%* of U(U, ?, U/K), 3.57%* of total, sub=0
2.U4a1(U)=1 14.28%* of U(U, ?, U/K), 3.57%* of total, sub=0
1.T(T)=7 25%* of total, sub=7
2.T1(T)=4 57.14%* of T(T), 14.28%* of total, sub=3
3.T1a(T)=3 100% of T1(T), 57.14% of T(T), 14.28% of total, sub=0
2.T2(T)=3 42.8%* of T(T), 10.7%* of total, sub=3
3.T2c1(T)=2 66.7%* of T2(T), 28.57%* of T(T), 7.14%* of total, sub=0
3.T2a1b(T)=1 33.3%* of T2(T), 14.28%* of T(T), 3.57%* of total, sub=0
1.K(K)=2 7.14%* of total, sub=0
1.J1 or J2b(J)=1 3.57%* of total, sub=0
1.N1a1a(N)=1 3.57%* of total, sub=0
1.X(X)=1 3.57%* of total, sub=0
1.W6(W)=1 3.57%* of total, sub=0
1.I1a(I)=1 3.57%* of total, sub=0
Pigmentation:
rs12913832=21: A/A=15(71.4%) A/G=4(19%) G/G=2(9.5%)
rs16891982=12: C/C=4(33.3%) C/G=3(25%) G/G=5(41.67%)
rs1042602=20: C/C=18(90%) C/A=1(5%) A/A=1(5%)
Samples from the Volga region
o KAL : Kalinovka I, Samara Oblast (Middle Volga), Russia
o NIK : Nikolaevka III, Samara Oblast (Middle Volga), Russia
o POD : Podlesnyj, Samara Oblast (Middle Volga), Russia
KAL1(EBA-Yam, Kalinovka, Russia (http://www.latlong.net/c/?lat=53.744075&long=50.248737))=N1a1a(N1a), has all of the defining HVR1 mutations of N1a1a but has one extra(16344T) that doesn't exist in any N1 subclades. Pigmentation: rs12913832 A/G, rs16891982 C/C, rs1042602 A/A.
KAL2(EBA-Yam, Kalinovka, Russia (http://www.latlong.net/c/?lat=53.744075&long=50.248737))=H(H), did not list any HVR1 mutations. Pigmentation: rs12913832 G/G.
NIK1(EBA-Yam, Nikolaevka III, Russia (http://www.latlong.net/c/?lat=53.125556&long=47.205833))=T1a(T), has all of the defining HVR1 mutations of T1a. Pigmentation: rs12913832 A/A, rs1042602 C/C.
NIK7(EBA-Yam, Nikolaevka III, Russia (http://www.latlong.net/c/?lat=53.125556&long=47.205833))=H(H), rCRS. Pigmentation: rs12913832 A/A, rs16891982 C/G, rs1042602 C/C.
POD1(EBA-Yam, Podlesnyj, Russia (http://www.latlong.net/c/?lat=53.281578&long=50.907795))=W6(W), has all of the defining HVR1 mutations of W6. Pigmentation: rs12913832 A/G, rs16891982 G/G, rs1042602 C/C.
POD2(EBA-Yam, Podlesnyj, Russia (http://www.latlong.net/c/?lat=53.281578&long=50.907795))=T2c1(T), has all of the defining HVR1 mutations of JT, T, and T2c1, but not the one for T2 but still may have had it. Pigmentation: rs12913832 A/A, rs16891982 G/G, rs1042602 C/C.
Samples from the Don and Kuban regions
o OLE : Olennii, Krasnodar Krai (Sea of Azov), Russia
o PEJ : Peschanyi, Rostov Oblast, Russia
OLE1(EBA-Yam, Olennii, Russia (http://www.latlong.net/c/?lat=45.641529&long=39.705598))=T2c1(T), has all of the defining HVR1 mutations of JT, T, and T2c1, but not the sole HVR1 defining mutation of T2(16296T) but still may have had it. Pigmentation: rs12913832 A/A, rs1042602 C/C.
OLE7(EBA-Yam, Olennii, Russia (http://www.latlong.net/c/?lat=45.641529&long=39.705598))=J1 or J2b(J), J1 and J2b have the same HVR1 defining mutations and OLE7 has all of them plus one extra HVR1 mutation(16048A), which does not exist in any JT subclades. No pigmentation alleles listed.
PEJ1(EBA-Yam, Peschanyi, Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=U5a1(U), has all of the HVR1 mutations of U5a1. Pigmentation: rs12913832 A/A, rs1042602 C/C.
Samples from central and eastern Ukraine
o PES : Pestchanka II, central eastern Ukraine
o SUG : Kirovograd Sugokleya, central Ukraine
o VIN : Vinogradnoe, southern central Ukraine
PES7(EBA-Yam, Pestchanka II, Ukraine (http://www.latlong.net/c/?lat=48.598536&long=35.314148))=H(H), has two HVR1 mutations: 16209C and 16042A. 16042A does not exist in any RO subclades but 16209C exists in two H subclades: H1a1 and H5a1j. Pigmentation: rs16891982 G/G, rs1042602 C/C.
SUG2(EBA-Yam, Kirovograd, Ukraine (http://www.latlong.net/c/?lat=48.522238&long=32.251308))=I1a(I), has all of the defining HVR1 mutations of I1a. Pigmentation: rs12913832 A/A, rs16891982 C/C, rs1042602 C/C.
SUG6(EBA-Yam, Kirovograd, Ukraine (http://www.latlong.net/c/?lat=48.522238&long=32.251308))=H(H), listed one HVR1 mutation which is a defining mutation in seven H subclades: H1a'b'c'd'h, H1c3b, H1bv1, H3ak, H6, H8, H13b1(16362C). Pigmentation: rs12913832 A/A, rs16891982 C/C, rs1042602 C/C.
SUG7(EBA-Yam, Kirovograd, Ukraine (http://www.latlong.net/c/?lat=48.522238&long=32.251308))=H(H), rCRS. No pigmentation alleles listed.
SUG8(EBA-Yam, Kirovograd, Ukraine (http://www.latlong.net/c/?lat=48.522238&long=32.251308))=H(H), rCRS. No pigmentation alleles listed.
VIN2(EBA-Yam, Vinogradnoe, Ukraine (http://www.latlong.net/c/?lat=47.334703&long=35.666093))=T1a(T), has all of the defining HVR1 mutations of T1a. Pigmentation: rs12913832 A/A, rs16891982 C/G, rs1042602 C/C.
VIN5(EBA-Yam, Vinogradnoe, Ukraine (http://www.latlong.net/c/?lat=47.334703&long=35.666093))=T1a(T), has all of the defining HVR1 mutations of T1a. Pigmentation: rs12913832 A/A.
VIN12(EBA-Yam, Vinogradnoe, Ukraine (http://www.latlong.net/c/?lat=47.334703&long=35.666093))=T1(T), has all of the defining HVR1 mutations of T1. Pigmentation: rs12913832 A/A, rs1042602 C/C.
Samples from western Ukraine & Moldova
o MAJ : Mayaki, southwest Ukraine
o TET : Tetcani, northern Moldova
MAJ3(EBA-Yam, Mayaki, Ukraine (http://www.latlong.net/c/?lat=46.413300&long=30.276532))=U5a1(?), has all of the defining HVR1 mutations of U5(except one but still may have had it), U5a, and U5a1. Pigmentation: rs12913832 A/A, rs1042602 C/C.
MAJ4(EBA-Yam, Mayaki, Ukraine (http://www.latlong.net/c/?lat=46.413300&long=30.276532))=U5(U), has one of two defining HVR1 mutations of U5(16192T) and U5 is the only U subclade with it. The other HVR1 mutation listed for MAJ4 is 16311C which is a defining mutation for seven U5 subclades: U5a1f1, U5a2e, U5b1c, U5b1i, U5b2a1a(16311C), U5b2a5, and U5b3d. Pigmentation: rs12913832 A/A, rs1042602 C/C.
MAJ5(EBA-Yam, Mayaki, Ukraine (http://www.latlong.net/c/?lat=46.413300&long=30.276532))=X(X), has all of the defining HVR1 mutations of X and one extra HVR1 mutation(16136C) which doesn't exist in any X subclades. Pigmentation: rs12913832 G/G, rs16891982 C/C, rs1042602 C/C.
TET2(EBA-Yam, Tetcani,Moldova (http://www.latlong.net/c/?lat=48.183333&long=26.983333))=U4a1(U), has all of the defining HVR1 mutations of U4 and U4a1. No pigmentation alleles listed.
Samples from Bulgaria
o OVI : Ovchartsi, south-east Bulgaria
o POP : Popovo, south-east Bulgaria
o RIL : Riltsi, north-east Bulgaria
OV12(EBA-Yam, Ovchartsi, Bulgaria (http://www.latlong.net/c/?lat=42.235930&long=26.083413))=K(K), has all of the defining HVR1 mutations of K plus one extra(16290T), which does not exist in any U subclades. No pigmentation alleles.
OV13(EBA-Yam, Ovchartsi, Bulgaria (http://www.latlong.net/c/?lat=42.235930&long=26.083413))=U/K(U/K), didn't list any HVR1 mutations. Pigmentation: rs12913832 A/G, rs1042602 C/C.
POP1(EBA-Yam, Popovo, Bulgaria (http://www.latlong.net/c/?lat=42.205594&long=26.736253))=T2a1b(T), has all of the defining HVR1 mutations of JT, T, T2, and T2a1b. Pigmentation: rs12913832 A/G, rs16891982 C/G, rs1042602 C/C.
POP3(EBA-Yam, Popovo, Bulgaria (http://www.latlong.net/c/?lat=42.205594&long=26.736253))=U2e(U), has all of the defining HVR1 mutations of U2 and U2e(2/3 may have had the missing one). Pigmentation: rs12913832 A/A, rs16891982 G/G, rs1042602 C/C.
POP4(EBA-Yam, Popovo, Bulgaria (http://www.latlong.net/c/?lat=42.205594&long=26.736253))=U5a1(U), has all of the defining HVR1 mutations of U5(except one but still may have had it), U5a, and U5a1. Pigmentation: rs12913832 A/A, rs1042602 C/C.
RIL3(EBA-Yam, Riltsi, Bulgaria (http://www.latlong.net/c/?lat=43.594204&long=27.778568))=K(K), has both of the needed HVR1 mutations to be K but also has HVR1 mutation 16192T and U5 is the only U subclade with it. Pigmentation: rs12913832 A/A, rs16891982 G/G, rs1042602 C/A.
EBA-Cat1(4,700-4,500BP) and EBA-Cat2(4,500-4,000BP)
mtDNA=27
1.U(U, U/K)=12 44.4%* of total, sub=11
2.U(U), 16356C=7 63.6% of U, 28.28% of total, sub=0
2.U5a1(U, U/K)=4 36.36% of U(U), 16.16% of total, sub=0
1.H(H)=7 26%* of total, sub=6(H-rCRS=1)
2.H(H), 16362C=4 66.7% of H(H), 17.28% of total, sub=0
2.H(H), 16234T=1 16.7% of H(H), 4.3% of total, sub=0
2.H2a1 or H(H), 16354T=1 16.7% of H(H), 4.3% of total, sub=0
1.J(J)=3 11.1%*, sub=3
2.J1b1a1(J)=2 66.7%* of J(J), 7.4%* of total, sub=0
2.J1 or J2b(J)=1 33.3%* of J(J), 3.7%* of total, sub=0
1.C4a=3 11.1%* of total, sub=3
2.C4a6=2 66.7%* of C4a, 7.4%* of total, sub=0
2.C4a3=1 33.3%* of C4a, 3.7%* of total, sub=0
1.R1(R1)=2 7.4%* of total, sub=0
1.I(I)=1 3.7%* of total, sub=0
Pigmentation:
rs12913832=22: A/A=17(77%), A/G=3(13.6%), G/G=2(9.1%)
rs16891982=8: C/C=4(50%), C/G= 3(37.5%), G/G=1(12.5%)
rs1042602=27: C/C=26(96.3%), C/A=1(3.7%), A/A=0(0%)
Samples from central and eastern Ukraine
o KNO : Krasnorechensk, eastern Ukraine
o LIS : Lisichansk, eastern Ukraine
o NEV : Nevskoe, eastern Ukraine
o NOZ : Novozvanovka II, eastern Ukraine
o SAC : Shakhta Stepnaya, eastern Ukraine
o SUG : Kirovograd Sugokleya, central Ukraine
o VIN : Vinogradnoe, southern central Ukraine
KNO4(EBA-Cat1, Krasnorechensk, Ukraine (http://www.latlong.net/c/?lat=49.210278&long=38.207500))=U(U), listed one HVR1 mutation(16356C) which is a defining HVR1 mutation in four U subclades: U5b1i, U2e3, U3a1c, and U4. Pigmentation: rs12913832 A/G, rs1042602 C/C.
LIS1(EBA-Cat1, Lisichansk, Ukraine (http://www.latlong.net/c/?lat=48.913697&long=38.434641))=U5a1(U), has all of the defining HVR1 mutations of U5(except one but still may have had it), U5a, and U5a1. Pigmentation: rs12913832 A/A, rs1042602 C/C.
LIS2(EBA-Cat1, Lisichansk, Ukraine (http://www.latlong.net/c/?lat=48.913697&long=38.434641))=U(U), listed one HVR1 mutation(16356C) which is a defining HVR1 mutation in four U subclades: U5b1i, U2e3, U3a1c, and U4. Pigmentation: rs12913832 A/A, rs1042602 C/C.
LIS3(EBA-Cat1, Lisichansk, Ukraine (http://www.latlong.net/c/?lat=48.913697&long=38.434641))=H2a1 or H(H), listed one HVR1 mutation(16354T) which is a defining mutation in only one H subclade, H2a1. Pigmentation: rs12913832 A/G, rs16891982 C/C, rs1042602 C/C.
NEV1(EBA-Cat2, Nevskoe, Ukraine (http://www.latlong.net/c/?lat=49.170017&long=37.975837))=U5a1(U), has all of the HVR1 mutations of U5a1. Pigmentation: rs12913832 A/A, rs16891982 C/C, rs1042602 C/C.
NEV3(EBA-Cat2, Nevskoe, Ukraine (http://www.latlong.net/c/?lat=49.170017&long=37.975837))=H(H), listed one HVR1 mutation which is a defining mutation in seven H subclades: H1a'b'c'd'h, H1c3b, H1bv1, H3ak, H6, H8, H13b1(16362C). Pigmentation: rs12913832 A/A, rs1042602 C/C.
NOZ1(EBA-Cat2, Novozvanovka II, Ukraine (http://www.latlong.net/c/?lat=48.583056&long=38.356111))=U(U), listed one HVR1 mutation(16356C) which is a defining HVR1 mutation in four U subclades: U5b1i, U2e3, U3a1c, and U4. Pigmentation: rs12913832 G/G, rs16891982 C/G, rs1042602 C/C.
NOZ2(EBA-Cat2, Novozvanovka II, Ukraine (http://www.latlong.net/c/?lat=48.583056&long=38.356111)):=U(U), listed one HVR1 mutation(16356C) which is a defining HVR1 mutation in four U subclades: U5b1i, U2e3, U3a1c, and U4. Pigmentation: rs12913832 G/G, rs1042602 C/C.
SAC2(EBA-Cat1, Shakhta Stepnaya, Ukraine (http://www.latlong.net/c/?lat=48.418717&long=36.429969))=J1 or J2b(J), J1 and J2b have the same HVR1 defining mutations and SAC2 had all of them. Pigmentation: rs12913832 A/A, rs1042602 C/C.
SUG5(EBA-Cat2, Kirovograd, Ukraine (http://www.latlong.net/c/?lat=48.522238&long=32.251308))=H(H), listed one HVR1 mutation which is a defining mutation in seven H subclades: H1a'b'c'd'h, H1c3b, H1bv1, H3ak, H6, H8, H13b1(16362C). Pigmentation: rs12913832 A/A, rs1042602 C/C.
VIN3(EBA-Cat2, Vinogradnoe, Ukraine (http://www.latlong.net/c/?lat=47.334703&long=35.666093))=U5a1(U/K), has all of the defining HVR1 mutations of U5(except one but still may have had it), U5a, and U5a1. Pigmentation: rs12913832 A/A, rs16891982 C/G, rs1042602 C/C.
VIN8(EBA-Cat2, Vinogradnoe, Ukraine (http://www.latlong.net/c/?lat=47.334703&long=35.666093))=J1b1a1(J1), has all of the defining HVR1 mutations of J1b1a1. Pigmentation: rs12913832 A/A, rs16891982 C/C, rs1042602 C/A.
Samples from southern Russia
o PEJ : Peschanyi, Rostov Oblast, Russia
o TEM : Temrta, Rostov Oblast, Russia
PEJ2(EBA-Cat2, Peschanyi, Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=H(H), listed one HVR1 mutation(16234T) which is a defining HVR1 mutation in three H subclades: H1a1b, H13a1d, and H24a1. No pigmentation alleles listed.<o:p></o
PEJ3(EBA-Cat2, Peschanyi, Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=H(H), listed one HVR1 mutation which is a defining HVR1 mutation in seven H subclades: H1a'b'c'd'h, H1c3b, H1bv1, H3ak, H6, H8, H13b1(16362C). Pigmentation: rs12913832 A/A, rs1042602 C/C.
PEJ4(EBA-Cat2, Peschanyi, Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=H(H), listed one HVR1 mutation which is a defining HVR1 mutation in seven H subclades: H1a'b'c'd'h, H1c3b, H1bv1, H3ak, H6, H8, H13b1(16362C). Pigmentation: rs12913832 A/A, rs1042602 C/C.
PEJ5(EBA-Cat2, Peschanyi, Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=U(U), listed one HVR1 mutation(16356C) which is a defining HVR1 mutation in four U subclades: U5b1i, U2e3, U3a1c, and U4. Pigmentation: rs12913832 A/A, rs16891982 C/C, rs1042602 C/C.
TEM1(EBA-Cat2, Temrta Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=U(U), listed one HVR1 mutation(16356C) which is a defining HVR1 mutation in four U subclades: U5b1i, U2e3, U3a1c, and U4. Pigmentation: rs12913832 A/A, rs1042602 C/C.
TEM2(EBA-Cat1, Temrta Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=H(H), rCRS. Pigmentation: rs12913832 A/A, rs16891982 G/G, rs1042602 C/C.
TEM3(EBA-Cat2, Temrta Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=J1b1a1(J1), has all of the defining HVR1 mutations of J1b1a1. No pigmentation alleles listed.
TEM4(EBA-Cat1, Temrta Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=U5a1(U/K), has all of the defining HVR1 mutations of U5(except one but still may have had it), U5a, and U5a1. Pigmentation: rs12913832 A/A, rs16891982 C/G, rs1042602 C/C.
TEM5(EBA-Cat1, Temrta Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=R1(R1), TEM5 has one HVR1 mutation listed and is the sole HVR1 mutation which defines R1. Pigmentation: rs12913832 A/A, rs1042602 C/C.
TEM6(EBA-Cat1, Temrta Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=R1(R1), TEM6 has one HVR1 mutation listed and is the sole HVR1 mutation which defines R1. Pigmentation: rs12913832 A/A, rs1042602 C/C.
TEM7(EBA-Cat1, Temrta Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=U(U), listed one HVR1 mutation(16356C) which is a defining HVR1 mutation in four U subclades: U5b1i, U2e3, U3a1c, and U4. Pigmentation: rs12913832 A/A, rs1042602 C/C.
TEM8(EBA-Cat2, Temrta Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=U(U), did not list any HVR1 mutations. Pigmentation: rs12913832 A/G, rs1042602 C/C.
Samples from Moldova
o TET : Tetcani, northern Moldova
TET1(EBA-Cat2, Tetcani,Moldova (http://www.latlong.net/c/?lat=48.183333&long=26.983333))=I(I), has all of the defining HVR1 mutations of N1a1b(I can also be called N1a1b2), may have also had I HVR1 defining mutations. No pigmentation alleles listed.
Samples from southwest Ukraine
The following mtDNA samples from the Odessa province were tested by Newton, J.R. (2011) (http://scholarworks.gvsu.edu/theses/5/)
.
C4a3
C4a6 (x2)
Early ENL(6,500-5,000BP) and Late ENL(5,500-4,000BP)
mtDNA=10
1.H(H)=4 40%* of total, sub=4
2.H(H), rCRS=2 50%* of H(H), 20%* of total, sub=0
2.H5(H)=1 20%* of H(H), 10%* of total, sub=0
2.H(H), 16261T=1 20%* of H(H), 10%* of total, sub=0
1.U5a(U, U/k)=3 30%* of total, sub=3
2.U5a1(U, U/K)=2 66.7%* of U5a(U, U/K), 20%* of total, sub=0
2.U5a2a(U)=1 33.3%* of U5a(U, U/K), 10%* of total, sub=0
1.T2(T)=2 20%* of total, sub=2
2.T2b(T)=1 50%* of T2(T), 10%* of total, sub=0
2.T2e(T)=1 50%* of T2(T), 10%* of total, sub=0
1.W(W)=1 10%* of total, sub=0
Pigmentation:
rs12913832: A/A=4 A/G=0 G/G=0
rs16891982: C/C=1 C/G=1 G/G=0
rs1042602: C/C=4 C/A=0 A/A=0
Samples from Ukraine
o VIN : Vinogradnoe, south-eastern Ukraine
o MAJ : Mayaki, southern central Ukraine
o MOB: Molyukhov, central Ukraine
VIN1(Early ENL, Vinogradnoe, Ukraine (http://www.latlong.net/c/?lat=47.334703&long=35.666093))=H(H), rCRS. Pigmentation: rs12913832 A/A, rs16891982 C/G, rs1042602 C/C.
MAJ8(Late ENL, Mayaki, Ukraine (http://www.latlong.net/c/?lat=46.413300&long=30.276532))=T2b(T), has all of the defining HVR1 mutations of T2b. No pigmentation alleles listed.
MAJ9(Late ENL, Mayaki, Ukraine (http://www.latlong.net/c/?lat=46.413300&long=30.276532))=W(W), has all of the HVR1 defining mutations of W. Pigmentation: rs12913832 A/A, rs16891982 C/C, rs1042602 C/C.
.
MOB1(Late ENL, Molyukhov Bugor, Ukraine (http://www.latlong.net/c/?lat=49.115710&long=32.509085))=U5a1(U), has all of the HVR1 mutations of U5a1. Pigmentation: rs12913832 A/A, rs1042602 C/C.
MOB3(Late ENL, Molyukhov Bugor, Ukraine (http://www.latlong.net/c/?lat=49.115710&long=32.509085))=U5a1(U/K), has all of the HVR1 mutations of U5a1. No pigmentation alleles listed.
Samples from Bulgaria
o SMY : Smyadovo, eastern Bulgaria
o DUR : Durankulak, north-eastern Bulgaria
SMY3(Early ENL, Smyadovo, Bulgaria (http://www.latlong.net/c/?lat=43.051196&long=26.990642))=H, rCRS. No pigmentation alleles listed.
SMY4(Early ENL, Smyadovo, Bulgaria (http://www.latlong.net/c/?lat=43.051196&long=26.990642))=H5(H), listed one HVR1 mutation(16304C) and H5 is the only H subclade with it. No pigmentation alleles listed.
SMY9(Early ENL, Smyadovo, Bulgaria (http://www.latlong.net/c/?lat=43.051196&long=26.990642))=H(H), listed one HVR1 mutation(16261T) which exists in three H subclades: H1as1a, H7a1, H13a1a1d, and H13b1. Pigmentation: rs12913832 A/A, rs1042602 C/C.
SMY11(Early ENL, Smyadovo, Bulgaria (http://www.latlong.net/c/?lat=43.051196&long=26.990642))=T2e(T), has all of the defining HVR1 mutations of T2e but has one extra HVR1 mutation(16269G) which does not exist in any T subclades. No pigmentation alleles listed.
DUR1(Late ENL, Durankulak, Bulgaria (http://www.latlong.net/c/?lat=43.687143&long=28.531102))=U5a2a(U), has all of the defining HVR1 mutations of U5, U5a, not the single one for U5a2(may have had it), but both of the ones for U5a2a. No pigmentation alleles listed.
Map of these results. (https://mapsengine.google.com/map/edit?mid=zpAcd_2YNln8.kiAdXb0B2q5I) Many of the same rules apply, I suggest clicking on the name list on the left not on the map since many of them are on the exact same location so you can't view all of them.
How individuals info is setup: I put the name of the individual(EX: BEN3, SYM11, etc.) and in ()'s their Archaeological date(EX: EBA-Yam, EBA-Cat2, etc.) and location(found by plugging in latitude and longitude to this site (http://www.latlong.net/) and click here (https://mapsengine.google.com/map/edit?mid=zm0B1VqcS1ZE.kixFYqDlP5v8) for even more accurate locations). The first haplogroup listed is my prediction based on HVR1 mutations(aka differences to rCRS) given in Table.S1 of Wilde 2014 (http://www.ancestraljourneys.org/bibliography.shtml#Wilde2014). I used this predictor (http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&sqi=2&ved=0CC0QFjAA&url=http%3A%2F%2Fdna.jameslick.com%2F&ei=nB0kU-W2KoqsyAHKwIGoCw&usg=AFQjCNFGhJKVXY_TyAOJF2QQwvI2OBBd2A&sig2=75P8dZJIKZ90JZJQc4MSVQ&bvm=bv.62922401,d.aWc) and this mtDNA tree (http://www.phylotree.org/tree/main.htm)to make my predictions. The haplogroup in ()'s is what Wilde 2014 (http://www.ancestraljourneys.org/bibliography.shtml#Wilde2014) reported based on coding region mutations(aka differences to rCRS) in Table.S2, after that I give some reasoning why I predicted a certain haplogroup.
For every individual I put the pigmentation SNP's in this order rs12913832, rs16891982, rs1042602 because that is the order it is in in Table.S1 of Wilde 2014 (http://www.ancestraljourneys.org/bibliography.shtml#Wilde2014). rs12913832 (http://snpedia.com/index.php/Rs12913832) is associated with eye color, according to SNPedia A/A alleles is brown eye color 80% of the time, A/G is brown eye color, G/G is blue eye color 99% of the time. According to the 8-plex system (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3694299/)G/G can be any light color and A/G or A/A just mean not blue. rs16891982 (http://www.snpedia.com/index.php/Rs16891982)is associated with skin and hair color, according to SNPedia G/G alleles are associated with light skin and C/G or C/C in Europeans makes them 7x more likely to have black hair. G/G alleles are around 80% or more on average in Europeans and around 50% in near easterns(click here (http://mbe.oxfordjournals.org/content/24/3/710/F3.expansion.html)). According to Wikipedia rs1042602 (http://snpedia.com/index.php/Rs1042602) alleles G/G are associated with light skin and are about 40-50% in Europeans and near easterns(click here (http://mbe.oxfordjournals.org/content/24/3/710/F3.expansion.html)).
I split up the individuals according to their archeological date and within that their location. For sections EBA-Yam and EBA-Cat I copied the format, regions, and order of individuals from Maciamo at Eupedia.com(click here (http://www.eupedia.com/genetics/haplogroups_of_bronze_age_proto-indo-europeans.shtml)) and i also copied his format for the ENL section but not the regions and order of individuals. You can see that Maciamo's predictions based on HVR1 mutations are nearly identical to mine and Jean Manco made many of the same predictions at her site ancestral journeys(click here (http://www.ancestraljourneys.org/ancientdna.shtml)).
I strongly dis agree with some of Maciamo's predictions. Example: there are seven individuals in the archeological date EBA-Cat1 or EBA-Cat2 listed as having mtDNA haplogroup U(Table.S2) and with 16356C a their only HVR1 mutation. There are four U subclades with that mutation U5b1i, U2e3, U3a1c, and U4 and for every single one of those individuals Maciamo predicted U4. U4 makes the most sense but I think he needs to be more conservative. There are many other examples where I think Maciamo needed to be more conservative or more liberal.
For total mtDNA percentages I ordered them in numbers, 1. means it is not a subclade of any haplogroups listed, 2. its 1.'s subclade's 3. its 2.'s subclades and so on. The * means that its a independent percentage that isn't raised because its a subclade of a popular haplogroup without many subclades. In parenthesis I put the reported haplogroup according to coding region mutations.
EBA-Yam(5,000-4,500BP)
mtDNA=28
1.H(H)=7 25%* of total, sub=0
1.U(U, ?, U/K)=7 25%* of total, sub=7
2.U5(U, ?)=4 57%* of U(U, ?, U/K), 14.28%* of total, sub=3(U5(U)=1)
3.U5a1(U, ?)=3 100% of U5(U, ?), 57% of U(U, ?, U/K), 14.28% of total, sub=0(U5a1(?)=1, U5a1(U)=2)
2.U/K(U/K)=1 14.28%* of U(U, ?, U/K), 3.57%* of total, sub=0
2.U2e(U)=1 14.28%* of U(U, ?, U/K), 3.57%* of total, sub=0
2.U4a1(U)=1 14.28%* of U(U, ?, U/K), 3.57%* of total, sub=0
1.T(T)=7 25%* of total, sub=7
2.T1(T)=4 57.14%* of T(T), 14.28%* of total, sub=3
3.T1a(T)=3 100% of T1(T), 57.14% of T(T), 14.28% of total, sub=0
2.T2(T)=3 42.8%* of T(T), 10.7%* of total, sub=3
3.T2c1(T)=2 66.7%* of T2(T), 28.57%* of T(T), 7.14%* of total, sub=0
3.T2a1b(T)=1 33.3%* of T2(T), 14.28%* of T(T), 3.57%* of total, sub=0
1.K(K)=2 7.14%* of total, sub=0
1.J1 or J2b(J)=1 3.57%* of total, sub=0
1.N1a1a(N)=1 3.57%* of total, sub=0
1.X(X)=1 3.57%* of total, sub=0
1.W6(W)=1 3.57%* of total, sub=0
1.I1a(I)=1 3.57%* of total, sub=0
Pigmentation:
rs12913832=21: A/A=15(71.4%) A/G=4(19%) G/G=2(9.5%)
rs16891982=12: C/C=4(33.3%) C/G=3(25%) G/G=5(41.67%)
rs1042602=20: C/C=18(90%) C/A=1(5%) A/A=1(5%)
Samples from the Volga region
o KAL : Kalinovka I, Samara Oblast (Middle Volga), Russia
o NIK : Nikolaevka III, Samara Oblast (Middle Volga), Russia
o POD : Podlesnyj, Samara Oblast (Middle Volga), Russia
KAL1(EBA-Yam, Kalinovka, Russia (http://www.latlong.net/c/?lat=53.744075&long=50.248737))=N1a1a(N1a), has all of the defining HVR1 mutations of N1a1a but has one extra(16344T) that doesn't exist in any N1 subclades. Pigmentation: rs12913832 A/G, rs16891982 C/C, rs1042602 A/A.
KAL2(EBA-Yam, Kalinovka, Russia (http://www.latlong.net/c/?lat=53.744075&long=50.248737))=H(H), did not list any HVR1 mutations. Pigmentation: rs12913832 G/G.
NIK1(EBA-Yam, Nikolaevka III, Russia (http://www.latlong.net/c/?lat=53.125556&long=47.205833))=T1a(T), has all of the defining HVR1 mutations of T1a. Pigmentation: rs12913832 A/A, rs1042602 C/C.
NIK7(EBA-Yam, Nikolaevka III, Russia (http://www.latlong.net/c/?lat=53.125556&long=47.205833))=H(H), rCRS. Pigmentation: rs12913832 A/A, rs16891982 C/G, rs1042602 C/C.
POD1(EBA-Yam, Podlesnyj, Russia (http://www.latlong.net/c/?lat=53.281578&long=50.907795))=W6(W), has all of the defining HVR1 mutations of W6. Pigmentation: rs12913832 A/G, rs16891982 G/G, rs1042602 C/C.
POD2(EBA-Yam, Podlesnyj, Russia (http://www.latlong.net/c/?lat=53.281578&long=50.907795))=T2c1(T), has all of the defining HVR1 mutations of JT, T, and T2c1, but not the one for T2 but still may have had it. Pigmentation: rs12913832 A/A, rs16891982 G/G, rs1042602 C/C.
Samples from the Don and Kuban regions
o OLE : Olennii, Krasnodar Krai (Sea of Azov), Russia
o PEJ : Peschanyi, Rostov Oblast, Russia
OLE1(EBA-Yam, Olennii, Russia (http://www.latlong.net/c/?lat=45.641529&long=39.705598))=T2c1(T), has all of the defining HVR1 mutations of JT, T, and T2c1, but not the sole HVR1 defining mutation of T2(16296T) but still may have had it. Pigmentation: rs12913832 A/A, rs1042602 C/C.
OLE7(EBA-Yam, Olennii, Russia (http://www.latlong.net/c/?lat=45.641529&long=39.705598))=J1 or J2b(J), J1 and J2b have the same HVR1 defining mutations and OLE7 has all of them plus one extra HVR1 mutation(16048A), which does not exist in any JT subclades. No pigmentation alleles listed.
PEJ1(EBA-Yam, Peschanyi, Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=U5a1(U), has all of the HVR1 mutations of U5a1. Pigmentation: rs12913832 A/A, rs1042602 C/C.
Samples from central and eastern Ukraine
o PES : Pestchanka II, central eastern Ukraine
o SUG : Kirovograd Sugokleya, central Ukraine
o VIN : Vinogradnoe, southern central Ukraine
PES7(EBA-Yam, Pestchanka II, Ukraine (http://www.latlong.net/c/?lat=48.598536&long=35.314148))=H(H), has two HVR1 mutations: 16209C and 16042A. 16042A does not exist in any RO subclades but 16209C exists in two H subclades: H1a1 and H5a1j. Pigmentation: rs16891982 G/G, rs1042602 C/C.
SUG2(EBA-Yam, Kirovograd, Ukraine (http://www.latlong.net/c/?lat=48.522238&long=32.251308))=I1a(I), has all of the defining HVR1 mutations of I1a. Pigmentation: rs12913832 A/A, rs16891982 C/C, rs1042602 C/C.
SUG6(EBA-Yam, Kirovograd, Ukraine (http://www.latlong.net/c/?lat=48.522238&long=32.251308))=H(H), listed one HVR1 mutation which is a defining mutation in seven H subclades: H1a'b'c'd'h, H1c3b, H1bv1, H3ak, H6, H8, H13b1(16362C). Pigmentation: rs12913832 A/A, rs16891982 C/C, rs1042602 C/C.
SUG7(EBA-Yam, Kirovograd, Ukraine (http://www.latlong.net/c/?lat=48.522238&long=32.251308))=H(H), rCRS. No pigmentation alleles listed.
SUG8(EBA-Yam, Kirovograd, Ukraine (http://www.latlong.net/c/?lat=48.522238&long=32.251308))=H(H), rCRS. No pigmentation alleles listed.
VIN2(EBA-Yam, Vinogradnoe, Ukraine (http://www.latlong.net/c/?lat=47.334703&long=35.666093))=T1a(T), has all of the defining HVR1 mutations of T1a. Pigmentation: rs12913832 A/A, rs16891982 C/G, rs1042602 C/C.
VIN5(EBA-Yam, Vinogradnoe, Ukraine (http://www.latlong.net/c/?lat=47.334703&long=35.666093))=T1a(T), has all of the defining HVR1 mutations of T1a. Pigmentation: rs12913832 A/A.
VIN12(EBA-Yam, Vinogradnoe, Ukraine (http://www.latlong.net/c/?lat=47.334703&long=35.666093))=T1(T), has all of the defining HVR1 mutations of T1. Pigmentation: rs12913832 A/A, rs1042602 C/C.
Samples from western Ukraine & Moldova
o MAJ : Mayaki, southwest Ukraine
o TET : Tetcani, northern Moldova
MAJ3(EBA-Yam, Mayaki, Ukraine (http://www.latlong.net/c/?lat=46.413300&long=30.276532))=U5a1(?), has all of the defining HVR1 mutations of U5(except one but still may have had it), U5a, and U5a1. Pigmentation: rs12913832 A/A, rs1042602 C/C.
MAJ4(EBA-Yam, Mayaki, Ukraine (http://www.latlong.net/c/?lat=46.413300&long=30.276532))=U5(U), has one of two defining HVR1 mutations of U5(16192T) and U5 is the only U subclade with it. The other HVR1 mutation listed for MAJ4 is 16311C which is a defining mutation for seven U5 subclades: U5a1f1, U5a2e, U5b1c, U5b1i, U5b2a1a(16311C), U5b2a5, and U5b3d. Pigmentation: rs12913832 A/A, rs1042602 C/C.
MAJ5(EBA-Yam, Mayaki, Ukraine (http://www.latlong.net/c/?lat=46.413300&long=30.276532))=X(X), has all of the defining HVR1 mutations of X and one extra HVR1 mutation(16136C) which doesn't exist in any X subclades. Pigmentation: rs12913832 G/G, rs16891982 C/C, rs1042602 C/C.
TET2(EBA-Yam, Tetcani,Moldova (http://www.latlong.net/c/?lat=48.183333&long=26.983333))=U4a1(U), has all of the defining HVR1 mutations of U4 and U4a1. No pigmentation alleles listed.
Samples from Bulgaria
o OVI : Ovchartsi, south-east Bulgaria
o POP : Popovo, south-east Bulgaria
o RIL : Riltsi, north-east Bulgaria
OV12(EBA-Yam, Ovchartsi, Bulgaria (http://www.latlong.net/c/?lat=42.235930&long=26.083413))=K(K), has all of the defining HVR1 mutations of K plus one extra(16290T), which does not exist in any U subclades. No pigmentation alleles.
OV13(EBA-Yam, Ovchartsi, Bulgaria (http://www.latlong.net/c/?lat=42.235930&long=26.083413))=U/K(U/K), didn't list any HVR1 mutations. Pigmentation: rs12913832 A/G, rs1042602 C/C.
POP1(EBA-Yam, Popovo, Bulgaria (http://www.latlong.net/c/?lat=42.205594&long=26.736253))=T2a1b(T), has all of the defining HVR1 mutations of JT, T, T2, and T2a1b. Pigmentation: rs12913832 A/G, rs16891982 C/G, rs1042602 C/C.
POP3(EBA-Yam, Popovo, Bulgaria (http://www.latlong.net/c/?lat=42.205594&long=26.736253))=U2e(U), has all of the defining HVR1 mutations of U2 and U2e(2/3 may have had the missing one). Pigmentation: rs12913832 A/A, rs16891982 G/G, rs1042602 C/C.
POP4(EBA-Yam, Popovo, Bulgaria (http://www.latlong.net/c/?lat=42.205594&long=26.736253))=U5a1(U), has all of the defining HVR1 mutations of U5(except one but still may have had it), U5a, and U5a1. Pigmentation: rs12913832 A/A, rs1042602 C/C.
RIL3(EBA-Yam, Riltsi, Bulgaria (http://www.latlong.net/c/?lat=43.594204&long=27.778568))=K(K), has both of the needed HVR1 mutations to be K but also has HVR1 mutation 16192T and U5 is the only U subclade with it. Pigmentation: rs12913832 A/A, rs16891982 G/G, rs1042602 C/A.
EBA-Cat1(4,700-4,500BP) and EBA-Cat2(4,500-4,000BP)
mtDNA=27
1.U(U, U/K)=12 44.4%* of total, sub=11
2.U(U), 16356C=7 63.6% of U, 28.28% of total, sub=0
2.U5a1(U, U/K)=4 36.36% of U(U), 16.16% of total, sub=0
1.H(H)=7 26%* of total, sub=6(H-rCRS=1)
2.H(H), 16362C=4 66.7% of H(H), 17.28% of total, sub=0
2.H(H), 16234T=1 16.7% of H(H), 4.3% of total, sub=0
2.H2a1 or H(H), 16354T=1 16.7% of H(H), 4.3% of total, sub=0
1.J(J)=3 11.1%*, sub=3
2.J1b1a1(J)=2 66.7%* of J(J), 7.4%* of total, sub=0
2.J1 or J2b(J)=1 33.3%* of J(J), 3.7%* of total, sub=0
1.C4a=3 11.1%* of total, sub=3
2.C4a6=2 66.7%* of C4a, 7.4%* of total, sub=0
2.C4a3=1 33.3%* of C4a, 3.7%* of total, sub=0
1.R1(R1)=2 7.4%* of total, sub=0
1.I(I)=1 3.7%* of total, sub=0
Pigmentation:
rs12913832=22: A/A=17(77%), A/G=3(13.6%), G/G=2(9.1%)
rs16891982=8: C/C=4(50%), C/G= 3(37.5%), G/G=1(12.5%)
rs1042602=27: C/C=26(96.3%), C/A=1(3.7%), A/A=0(0%)
Samples from central and eastern Ukraine
o KNO : Krasnorechensk, eastern Ukraine
o LIS : Lisichansk, eastern Ukraine
o NEV : Nevskoe, eastern Ukraine
o NOZ : Novozvanovka II, eastern Ukraine
o SAC : Shakhta Stepnaya, eastern Ukraine
o SUG : Kirovograd Sugokleya, central Ukraine
o VIN : Vinogradnoe, southern central Ukraine
KNO4(EBA-Cat1, Krasnorechensk, Ukraine (http://www.latlong.net/c/?lat=49.210278&long=38.207500))=U(U), listed one HVR1 mutation(16356C) which is a defining HVR1 mutation in four U subclades: U5b1i, U2e3, U3a1c, and U4. Pigmentation: rs12913832 A/G, rs1042602 C/C.
LIS1(EBA-Cat1, Lisichansk, Ukraine (http://www.latlong.net/c/?lat=48.913697&long=38.434641))=U5a1(U), has all of the defining HVR1 mutations of U5(except one but still may have had it), U5a, and U5a1. Pigmentation: rs12913832 A/A, rs1042602 C/C.
LIS2(EBA-Cat1, Lisichansk, Ukraine (http://www.latlong.net/c/?lat=48.913697&long=38.434641))=U(U), listed one HVR1 mutation(16356C) which is a defining HVR1 mutation in four U subclades: U5b1i, U2e3, U3a1c, and U4. Pigmentation: rs12913832 A/A, rs1042602 C/C.
LIS3(EBA-Cat1, Lisichansk, Ukraine (http://www.latlong.net/c/?lat=48.913697&long=38.434641))=H2a1 or H(H), listed one HVR1 mutation(16354T) which is a defining mutation in only one H subclade, H2a1. Pigmentation: rs12913832 A/G, rs16891982 C/C, rs1042602 C/C.
NEV1(EBA-Cat2, Nevskoe, Ukraine (http://www.latlong.net/c/?lat=49.170017&long=37.975837))=U5a1(U), has all of the HVR1 mutations of U5a1. Pigmentation: rs12913832 A/A, rs16891982 C/C, rs1042602 C/C.
NEV3(EBA-Cat2, Nevskoe, Ukraine (http://www.latlong.net/c/?lat=49.170017&long=37.975837))=H(H), listed one HVR1 mutation which is a defining mutation in seven H subclades: H1a'b'c'd'h, H1c3b, H1bv1, H3ak, H6, H8, H13b1(16362C). Pigmentation: rs12913832 A/A, rs1042602 C/C.
NOZ1(EBA-Cat2, Novozvanovka II, Ukraine (http://www.latlong.net/c/?lat=48.583056&long=38.356111))=U(U), listed one HVR1 mutation(16356C) which is a defining HVR1 mutation in four U subclades: U5b1i, U2e3, U3a1c, and U4. Pigmentation: rs12913832 G/G, rs16891982 C/G, rs1042602 C/C.
NOZ2(EBA-Cat2, Novozvanovka II, Ukraine (http://www.latlong.net/c/?lat=48.583056&long=38.356111)):=U(U), listed one HVR1 mutation(16356C) which is a defining HVR1 mutation in four U subclades: U5b1i, U2e3, U3a1c, and U4. Pigmentation: rs12913832 G/G, rs1042602 C/C.
SAC2(EBA-Cat1, Shakhta Stepnaya, Ukraine (http://www.latlong.net/c/?lat=48.418717&long=36.429969))=J1 or J2b(J), J1 and J2b have the same HVR1 defining mutations and SAC2 had all of them. Pigmentation: rs12913832 A/A, rs1042602 C/C.
SUG5(EBA-Cat2, Kirovograd, Ukraine (http://www.latlong.net/c/?lat=48.522238&long=32.251308))=H(H), listed one HVR1 mutation which is a defining mutation in seven H subclades: H1a'b'c'd'h, H1c3b, H1bv1, H3ak, H6, H8, H13b1(16362C). Pigmentation: rs12913832 A/A, rs1042602 C/C.
VIN3(EBA-Cat2, Vinogradnoe, Ukraine (http://www.latlong.net/c/?lat=47.334703&long=35.666093))=U5a1(U/K), has all of the defining HVR1 mutations of U5(except one but still may have had it), U5a, and U5a1. Pigmentation: rs12913832 A/A, rs16891982 C/G, rs1042602 C/C.
VIN8(EBA-Cat2, Vinogradnoe, Ukraine (http://www.latlong.net/c/?lat=47.334703&long=35.666093))=J1b1a1(J1), has all of the defining HVR1 mutations of J1b1a1. Pigmentation: rs12913832 A/A, rs16891982 C/C, rs1042602 C/A.
Samples from southern Russia
o PEJ : Peschanyi, Rostov Oblast, Russia
o TEM : Temrta, Rostov Oblast, Russia
PEJ2(EBA-Cat2, Peschanyi, Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=H(H), listed one HVR1 mutation(16234T) which is a defining HVR1 mutation in three H subclades: H1a1b, H13a1d, and H24a1. No pigmentation alleles listed.<o:p></o
PEJ3(EBA-Cat2, Peschanyi, Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=H(H), listed one HVR1 mutation which is a defining HVR1 mutation in seven H subclades: H1a'b'c'd'h, H1c3b, H1bv1, H3ak, H6, H8, H13b1(16362C). Pigmentation: rs12913832 A/A, rs1042602 C/C.
PEJ4(EBA-Cat2, Peschanyi, Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=H(H), listed one HVR1 mutation which is a defining HVR1 mutation in seven H subclades: H1a'b'c'd'h, H1c3b, H1bv1, H3ak, H6, H8, H13b1(16362C). Pigmentation: rs12913832 A/A, rs1042602 C/C.
PEJ5(EBA-Cat2, Peschanyi, Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=U(U), listed one HVR1 mutation(16356C) which is a defining HVR1 mutation in four U subclades: U5b1i, U2e3, U3a1c, and U4. Pigmentation: rs12913832 A/A, rs16891982 C/C, rs1042602 C/C.
TEM1(EBA-Cat2, Temrta Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=U(U), listed one HVR1 mutation(16356C) which is a defining HVR1 mutation in four U subclades: U5b1i, U2e3, U3a1c, and U4. Pigmentation: rs12913832 A/A, rs1042602 C/C.
TEM2(EBA-Cat1, Temrta Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=H(H), rCRS. Pigmentation: rs12913832 A/A, rs16891982 G/G, rs1042602 C/C.
TEM3(EBA-Cat2, Temrta Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=J1b1a1(J1), has all of the defining HVR1 mutations of J1b1a1. No pigmentation alleles listed.
TEM4(EBA-Cat1, Temrta Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=U5a1(U/K), has all of the defining HVR1 mutations of U5(except one but still may have had it), U5a, and U5a1. Pigmentation: rs12913832 A/A, rs16891982 C/G, rs1042602 C/C.
TEM5(EBA-Cat1, Temrta Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=R1(R1), TEM5 has one HVR1 mutation listed and is the sole HVR1 mutation which defines R1. Pigmentation: rs12913832 A/A, rs1042602 C/C.
TEM6(EBA-Cat1, Temrta Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=R1(R1), TEM6 has one HVR1 mutation listed and is the sole HVR1 mutation which defines R1. Pigmentation: rs12913832 A/A, rs1042602 C/C.
TEM7(EBA-Cat1, Temrta Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=U(U), listed one HVR1 mutation(16356C) which is a defining HVR1 mutation in four U subclades: U5b1i, U2e3, U3a1c, and U4. Pigmentation: rs12913832 A/A, rs1042602 C/C.
TEM8(EBA-Cat2, Temrta Russia (http://www.latlong.net/c/?lat=46.563056&long=43.656667))=U(U), did not list any HVR1 mutations. Pigmentation: rs12913832 A/G, rs1042602 C/C.
Samples from Moldova
o TET : Tetcani, northern Moldova
TET1(EBA-Cat2, Tetcani,Moldova (http://www.latlong.net/c/?lat=48.183333&long=26.983333))=I(I), has all of the defining HVR1 mutations of N1a1b(I can also be called N1a1b2), may have also had I HVR1 defining mutations. No pigmentation alleles listed.
Samples from southwest Ukraine
The following mtDNA samples from the Odessa province were tested by Newton, J.R. (2011) (http://scholarworks.gvsu.edu/theses/5/)
.
C4a3
C4a6 (x2)
Early ENL(6,500-5,000BP) and Late ENL(5,500-4,000BP)
mtDNA=10
1.H(H)=4 40%* of total, sub=4
2.H(H), rCRS=2 50%* of H(H), 20%* of total, sub=0
2.H5(H)=1 20%* of H(H), 10%* of total, sub=0
2.H(H), 16261T=1 20%* of H(H), 10%* of total, sub=0
1.U5a(U, U/k)=3 30%* of total, sub=3
2.U5a1(U, U/K)=2 66.7%* of U5a(U, U/K), 20%* of total, sub=0
2.U5a2a(U)=1 33.3%* of U5a(U, U/K), 10%* of total, sub=0
1.T2(T)=2 20%* of total, sub=2
2.T2b(T)=1 50%* of T2(T), 10%* of total, sub=0
2.T2e(T)=1 50%* of T2(T), 10%* of total, sub=0
1.W(W)=1 10%* of total, sub=0
Pigmentation:
rs12913832: A/A=4 A/G=0 G/G=0
rs16891982: C/C=1 C/G=1 G/G=0
rs1042602: C/C=4 C/A=0 A/A=0
Samples from Ukraine
o VIN : Vinogradnoe, south-eastern Ukraine
o MAJ : Mayaki, southern central Ukraine
o MOB: Molyukhov, central Ukraine
VIN1(Early ENL, Vinogradnoe, Ukraine (http://www.latlong.net/c/?lat=47.334703&long=35.666093))=H(H), rCRS. Pigmentation: rs12913832 A/A, rs16891982 C/G, rs1042602 C/C.
MAJ8(Late ENL, Mayaki, Ukraine (http://www.latlong.net/c/?lat=46.413300&long=30.276532))=T2b(T), has all of the defining HVR1 mutations of T2b. No pigmentation alleles listed.
MAJ9(Late ENL, Mayaki, Ukraine (http://www.latlong.net/c/?lat=46.413300&long=30.276532))=W(W), has all of the HVR1 defining mutations of W. Pigmentation: rs12913832 A/A, rs16891982 C/C, rs1042602 C/C.
.
MOB1(Late ENL, Molyukhov Bugor, Ukraine (http://www.latlong.net/c/?lat=49.115710&long=32.509085))=U5a1(U), has all of the HVR1 mutations of U5a1. Pigmentation: rs12913832 A/A, rs1042602 C/C.
MOB3(Late ENL, Molyukhov Bugor, Ukraine (http://www.latlong.net/c/?lat=49.115710&long=32.509085))=U5a1(U/K), has all of the HVR1 mutations of U5a1. No pigmentation alleles listed.
Samples from Bulgaria
o SMY : Smyadovo, eastern Bulgaria
o DUR : Durankulak, north-eastern Bulgaria
SMY3(Early ENL, Smyadovo, Bulgaria (http://www.latlong.net/c/?lat=43.051196&long=26.990642))=H, rCRS. No pigmentation alleles listed.
SMY4(Early ENL, Smyadovo, Bulgaria (http://www.latlong.net/c/?lat=43.051196&long=26.990642))=H5(H), listed one HVR1 mutation(16304C) and H5 is the only H subclade with it. No pigmentation alleles listed.
SMY9(Early ENL, Smyadovo, Bulgaria (http://www.latlong.net/c/?lat=43.051196&long=26.990642))=H(H), listed one HVR1 mutation(16261T) which exists in three H subclades: H1as1a, H7a1, H13a1a1d, and H13b1. Pigmentation: rs12913832 A/A, rs1042602 C/C.
SMY11(Early ENL, Smyadovo, Bulgaria (http://www.latlong.net/c/?lat=43.051196&long=26.990642))=T2e(T), has all of the defining HVR1 mutations of T2e but has one extra HVR1 mutation(16269G) which does not exist in any T subclades. No pigmentation alleles listed.
DUR1(Late ENL, Durankulak, Bulgaria (http://www.latlong.net/c/?lat=43.687143&long=28.531102))=U5a2a(U), has all of the defining HVR1 mutations of U5, U5a, not the single one for U5a2(may have had it), but both of the ones for U5a2a. No pigmentation alleles listed.