Argentano
11-15-2016, 02:13 PM
Autosomal DNA of Argentine Mennonites
http://www.nature.com/articles/srep36392
Methods
Sampling
A total of 27 saliva samples were recruited on Oragene DNA collection kits (DNAgenotek) from individuals belonging to the Mennonite congregation of ‘La Nueva Esperanza’ (La Pampa, Argentina). Non related males from this DNA collection were previously analyzed for Y-chromosome markers.
Admixture analysis of Mennonite genomic profiles
Admixture analysis was carried out using a selection of 1000G population sets that represent the main continental ancestries. With the selected populations, K = 4 yielded the analysis with the lowest cross validation value; this analysis classifies profiles into four main groups that coincide with Europeans, Native Americans (represented here by Peruvians), East Asians, and Sub-Saharan Africans (Fig. 3A). This analysis allocates most of the Mennonite genomic variation (99.7% on average) to European ancestry.
http://www.nature.com/article-assets/npg/srep/2016/161108/srep36392/images_hires/w926/srep36392-f3.jpg
(A) Analysis of admixture of Mennonites using ADMIXTURE. The bar-plot represents individual ancestries in reference populations and Mennonites. The analysis was carried out using the unsupervised clustering algorithm implemented in ADMIXTURE and considering the run with the lowest cross validation value. (B) Genomic (chromosome) ancestry mosaic for one Mennonite (#MEN01) using PCAdmix (reference populations: CEU, YRI, CHB, PEL). (C) Admixture proportions of Mennonites using PCAdmix (reference populations: CEU, YRI, CHB, PEL).
La Pampa Menonnites
https://www.youtube.com/watch?v=CiQ9tn1TsBI
http://www.nature.com/articles/srep36392
Methods
Sampling
A total of 27 saliva samples were recruited on Oragene DNA collection kits (DNAgenotek) from individuals belonging to the Mennonite congregation of ‘La Nueva Esperanza’ (La Pampa, Argentina). Non related males from this DNA collection were previously analyzed for Y-chromosome markers.
Admixture analysis of Mennonite genomic profiles
Admixture analysis was carried out using a selection of 1000G population sets that represent the main continental ancestries. With the selected populations, K = 4 yielded the analysis with the lowest cross validation value; this analysis classifies profiles into four main groups that coincide with Europeans, Native Americans (represented here by Peruvians), East Asians, and Sub-Saharan Africans (Fig. 3A). This analysis allocates most of the Mennonite genomic variation (99.7% on average) to European ancestry.
http://www.nature.com/article-assets/npg/srep/2016/161108/srep36392/images_hires/w926/srep36392-f3.jpg
(A) Analysis of admixture of Mennonites using ADMIXTURE. The bar-plot represents individual ancestries in reference populations and Mennonites. The analysis was carried out using the unsupervised clustering algorithm implemented in ADMIXTURE and considering the run with the lowest cross validation value. (B) Genomic (chromosome) ancestry mosaic for one Mennonite (#MEN01) using PCAdmix (reference populations: CEU, YRI, CHB, PEL). (C) Admixture proportions of Mennonites using PCAdmix (reference populations: CEU, YRI, CHB, PEL).
La Pampa Menonnites
https://www.youtube.com/watch?v=CiQ9tn1TsBI