Nebula results soon

[Flashball]

I took the Nebula test for whole genome sequencing (30x) and will have the results soon

According to customer service, the status on the site is lagging behind what is in the lab.

"Thanks for getting back to us.

Apologies for the delay! There was a delay in updating the online tracker to check the progress of your sample. But I can confirm that your sample passed the QC phase. I assume that they are now preparing the reports so please allow us a few more days to complete the sequencing of your sample.

In the meantime, don't hesitate to reach us if you need any assistance. We appreciate your support and patience.


Kind regards,
Matt
Nebula Genomics Support"

https://image.noelshack.com/fichiers/2022/52/5/1672430482-321581342-724287585584194-3509130048483655521-n.png


I will post the results here.

[Flashball]

Update
https://imagizer.imageshack.com/img922/5013/rfAIJZ.jpg

[Beowulf]

Cool do they give Ancestry results too?

[Flashball]

Cool do they give Ancestry results too?

I don't know, it seems to me that they have something similar although not really ("deep ancestry"), but at the time they had a project with FTDNA for "NEBULA" version of "autosomal ancestry", but they had to give up for some reason

Anyway, what matters is the raw data you can use, the rest...

100% of your genome against 0.2% of your genome (23andme, Myheritage, LivingDna, etc.)

[Brás Garcia de Mascarenhas]

I have some interest in this test to be honest but there are two things that put me off. The first is the need to become a member and pay a monthly fee every month or get a lifetime membership for $275. The second is the 100 GB of Raw Data. How would you send a 100 GB file to Davidksi to generate your G25 for example, and does he have the capacity to process a file of that size?

[Flashball]

I have some interest in this test to be honest but there are two things that put me off. The first is the need to become a member and pay a monthly fee every month or get a lifetime membership for $275. The second is the 100 GB of Raw Data. How would you send a 100 GB file to Davidksi to generate your G25 for example, and does he have the capacity to process a file of that size?

I paid 165 euros (175 dollars) for a year of subscription to the site + the DNA test 30x complete genome sequencing... It depends on the periods, it is sometimes more expensive and sometimes less,
And I can cancel my subscription at any time, so it's not a problem


For the raw data, here is what customer service told me:

"We do sequence your entire genome and provide you all of the raw data! About 20,000 genes and ~40mil variants! The subscription (you selected yearly) provides access to the reports, traits, the aforementioned deep ancestry, and genome exploration tools. So you can look at any gene, condition or variants you might be interested in."


There is a converter too.

You can download your raw data of 300 MB and convert it in a gedmatch compatible format
https://youtu.be/HtEZZeLoOV0

https://www.dnagenics.com/article/wgs-to-raw

[Flashball]

https://www.wikitree.com/g2g/965923/can-nebula-genomics-data-be-used-here

"Nebula Genomics offers a free tool (a set of Python scripts) for converting the data format which they use (VCF) to the genealogical standard "23andMe" format (fields rsid, chromosome, position, genotype).

The script, and how to use it, is described and can be accessed here:

https://blog.nebula.org/how-to-start-exploring-your-raw-genomic-data/

For this to work, you'll need Python 3 installed on your computer. For simplicity, I'd recommend installing the Anaconda distribution of Python 3.7 (or whatever the current version). All of the commonly used optional features are included.

That 23andMe-style format (fields rsid, chromosome, position, genotype) should be compatible for sites including MyHeritage, DNA.land, Family Tree DNA, and other sites that permit users to upload DNA user data for cousin matching.

Having DNA listed on your profile is really just an aid for cousin matching (within those databases) and marking off DNA confirmation on the profiles of your mutual ancestors. "

[Brás Garcia de Mascarenhas]

I'll wait for your results and feedback then. I want to better understand what are the advantages and disadvantages of this company. I've been a little tired of always using the same data from 23andMe since 2017, it's time to test something different.

[Annihilus]

I took the Nebula test for whole genome sequencing (30x) and will have the results soon

According to customer service, the status on the site is lagging behind what is in the lab.

"Thanks for getting back to us.

Apologies for the delay! There was a delay in updating the online tracker to check the progress of your sample. But I can confirm that your sample passed the QC phase. I assume that they are now preparing the reports so please allow us a few more days to complete the sequencing of your sample.

In the meantime, don't hesitate to reach us if you need any assistance. We appreciate your support and patience.


Kind regards,
Matt
Nebula Genomics Support"

https://image.noelshack.com/fichiers/2022/52/5/1672430482-321581342-724287585584194-3509130048483655521-n.png


I will post the results here.

Nice. One question though, isn't genetic testing illigal in France?

[Flashball]

I'll wait for your results and feedback then. I want to better understand what are the advantages and disadvantages of this company.

That's what I want to show: If there are advantages and disadvantages I would say so and share them on this page.


Myheritage and 23andme recently stopped sending DNA kits to France, so for my brother I opted for full 30x Nebula sequencing (which I paid him)

But on this page you will have my results, not those of my brother.

If I can download my 300MB of raw data (which isn't huge) and convert it to a Gedmatch compatible file, that would be great enough.

Positive point: the customer service is very responsive.

[Flashball]

Nice. One question though, isn't genetic testing illigal in France?

It was already in 2016, when I ordered an FTDNA kit... but the government was more flexible? It seems (maybe) however that it is Myheritage who decided to stop for France, for 23andme I don't know.

Anyway, I'll post all the results here.

[Flashball]

https://image.noelshack.com/fichiers/2023/03/6/1674322118-nebularesults.png

[Flashball]

Y DNA:
I am R-FT288677 (u106 sub-branch)
As you can see I'm the ID YF114365 and there's another guy who has the same sub-branch (ID YF084402)
https://pbs.twimg.com/media/FnAARPfXEAEso8J?format=jpg&name=4096x4096


Maternal haplogroup:
https://pbs.twimg.com/media/Fm_-xndXoAIi1QV?format=jpg&name=large

[Flashball]

Good
50gb bam file converted into multitudes of raw data

The rar file is 1.5 GB in total, I'm downloading it right now


I don't expect to find my entire genome contained in a single raw data file, however I think there will likely be more SNPs in it than contained in the current raw data version of 23andme.
https://imagizer.imageshack.com/v2/706x1989q90/r/922/EkNAql.png

[HannibaltheGreat]

How is Yseq WGS++ conpared to Nebula?

[Flashball]

How is Yseq WGS++ conpared to Nebula?


I believe however considering the price, this is 100x whole genome sequencing, so it could be better than 30x (although that is already very good).

The difference can be located here I think:

"The YSEQ analysis does not contain any medical relevant information since the purpose is genealogy. But of course health related information is contained in the raw data and can be extracted from a third-party expert"

In the case of Nebula, there is information for health.. but it is relatively basic (not always), however they update via new studies (for certain diseases and others), which is very good .

The genome exploration interface on the Nebula site is also very good, relatively simple, very easy to find an SNP without going through the raw files.

In both cases, you can extract your raw data and derive more benefit from it, so don't worry.

I believe the price for 100x whole genome sequencing is about the same on Nebula, though you might find more promotion there I think, but that might be the case on yseq as well

Nebula customer service is pretty quick to respond, in my case I had a very active follow up. I had been told the steps, that some steps took longer than others (quality control much longer than sequencing, which only takes few day in my case).

I had no problems with Nebula, even when my kit took longer than expected to arrive: I contacted customer service and they answered me right away.



For YSEQ raw data extraction, there seems to be something more: they offer to convert your raw data into gedmatch (and others) compatible data, I guess?

You have to pay extra, but honestly, it would take less time to pay than doing it yourself (download 100-150 GB BAM). There is the VCF too, but I don't know if it's the same to convert a VCF to gedmatch compatible raw data, I had tried and the quality was lower than what 23andme offered, i.e. there were less Snps!!!

Nebula account offers this, it seems to me from what I understand, soon. Which will make the task easier, because it's very difficult to convert BAM files into gedmatch (and other) compatible files... I've already done it for a nuragic sample: I had to download a VCF, so not even a bam, and the quality was a little bit more bad after conversion.

[Zoro]

I have some interest in this test to be honest but there are two things that put me off. The first is the need to become a member and pay a monthly fee every month or get a lifetime membership for $275. The second is the 100 GB of Raw Data. How would you send a 100 GB file to Davidksi to generate your G25 for example, and does he have the capacity to process a file of that size?

If you are taking it just for sending it to Davidski or gedmatch you are wasting your money because Davidski will not use the whole WGS data because his test uses data from ancient data and other 23andme people. The ancient data is only 1250k and 23andme is like 700 or 800K so he will not be using the whole WGS data.
Same think gedmatch those calculators only use maximum 180K of data

So basically your results will change maybe little bit so maybe it is worth it a little bit. I think the only tests that use wgs are Eurasiandna.com but last i heard is they didnot have time for test for individual people

[Flashball]

If you are taking it just for sending it to Davidski or gedmatch you are wasting your money because Davidski will not use the whole WGS data because his test uses data from ancient data and other 23andme people. The ancient data is only 1250k and 23andme is like 700 or 800K so he will not be using the whole WGS data.
Same think gedmatch those calculators only use maximum 180K of data

So basically your results will change maybe little bit so maybe it is worth it a little bit. I think the only tests that use wgs are Eurasiandna.com but last i heard is they didnot have time for test for individual people

Yes, my converted Bam file contains a little more than a V3 23andme but that's it, don't expect a big difference on gedmatch...

I think it is not possible to exploit this kind of data with Gedmatch and G25... I mean, yes you can but that couldn't render fidelity to the high quality of your WSG data

"I think the only tests that use wgs are Eurasiandna.com" can you expand? how can they exploit large files?

[Etelfrido]

So, how did it compare to other companies you've tried?

[kingmob]

I'll wait for your results and feedback then. I want to better understand what are the advantages and disadvantages of this company. I've been a little tired of always using the same data from 23andMe since 2017, it's time to test something different.


The advantages are that you have medical reports on your whole genome, the list is endless, this is very useful for people who are interested in diseases/conditions they may suffer from.

For geneologists, you get good Y-chr coverage and you can upload your file to something like YFULL.

For anthrotards, the advantage is that you can produce a raw-dna file that has 100% SNP coverage with all gedmatch calculators.

The disadvantages are the pricing.

[calxpal]

Hi I'd like to know what else you've learned from it? :) Would you recommend it?

[kingmob]

Hi I'd like to know what else you've learned from it? :) Would you recommend it?


I recommend if you can afford it or find it on discount.

I have done nebula for one of my sons, my father and myself.