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View Full Version : Homo sapiens reached the higher latitudes of Europe by 45,000 years ago



Ajeje Brazorf
09-22-2024, 08:51 PM
https://www.nature.com/articles/s41586-023-06923-7

This study provides a detailed investigation into the presence of Homo sapiens in central and northwestern Europe around 45,000 years ago, focusing on the archaeological site of Ilsenhöhle in Ranis, Germany. The research team employed a combination of radiocarbon dating, proteomic analysis, and mitochondrial DNA (mtDNA) sequencing to directly associate Homo sapiens remains with the Lincombian-Ranisian-Jerzmanowician (LRJ) stone tool industry. This technocomplex is characterized by bifacial leaf points and Jerzmanowice blade points, and its attribution to either Neanderthals or Homo sapiens was previously debated.

Key findings include:

1. Radiocarbon Dating: Human remains from layers 9 and 8 at the Ranis site were directly dated to 47,500–43,260 calibrated years before present (cal BP). These dates make the remains some of the oldest directly dated Upper Palaeolithic H. sapiens remains in Eurasia. Six additional human bones from the 1930s excavation, thought to be associated with LRJ, were also directly dated and fit within the same time range.

2. Proteomic and Morphological Analysis: Proteomic methods (ZooMS and SPIN) identified 13 hominin bones from both the 1930s and the 2016–2022 excavations. These bones were from layers associated with the LRJ. Morphological analysis confirmed that the bones belonged to Homo sapiens rather than Neanderthals.

3. mtDNA Analysis: Mitochondrial DNA was recovered from 11 human skeletal fragments, all of which were identified as belonging to ancient Homo sapiens. Ten of these genomes were reconstructed to near-completion, and five showed no pairwise differences, suggesting they came from either the same individual or maternally related individuals. The mtDNA sequences clustered with other early H. sapiens individuals, such as the Zlatý kůň individual from the Czech Republic, which dates to around 45,000 years ago.

4. Technocomplex Attribution: The LRJ was previously thought to be possibly produced by Neanderthals due to its mix of bifacial and unifacial tool types, which included some Middle Palaeolithic features. However, this study definitively links the LRJ at Ranis to Homo sapiens based on the direct association of H. sapiens remains with LRJ stone tools. This finding supports the hypothesis that Homo sapiens were present in central and northwestern Europe during the Middle to Upper Palaeolithic transition, before Neanderthals became extinct in other regions.

5. Climate and Faunal Analysis: The study also incorporates zooarchaeological and sedimentological data to reconstruct the environmental conditions of the time. The presence of reindeer and other cold-adapted species, along with sediment analysis, suggests that H. sapiens occupied this region during a period of colder climate, with temperatures 7–15°C lower than present-day. This is consistent with a subarctic environment during Greenland Stadial 12 or 13 (around 45,000 years ago).

6. Implications for Neanderthal-Human Interaction: The research suggests that Homo sapiens were in Europe at the same time as the last Neanderthal populations, but that these groups were likely part of distinct populations with limited direct interaction in this region. This challenges earlier ideas of a strict chronological separation between Neanderthals and Homo sapiens in northwestern Europe, proposing instead a patchwork of different human groups with overlapping timelines.

In summary, this study provides strong evidence that Homo sapiens were responsible for the LRJ technocomplex and were present in central and northwestern Europe earlier than previously thought, coexisting with Neanderthals in other parts of Europe. The research offers new insights into the complexity of human population dynamics during the Middle to Upper Palaeolithic transition.

Ajeje Brazorf
09-22-2024, 08:52 PM
These samples are among those with the highest amount of Neanderthal DNA ever sequenced so far. Here, I have sorted them in descending order, from the most Neanderthal to the least.


Germany_Ilsenhöhle_LRJ_UP:RNI085__BC_42635__Cov_72 .88%,-0.093335,-0.06804,-0.089,0.049096,0.003693,-0.010598,-0.005405,-0.001615,0.029247,-0.007107,0.012666,-0.007493,-0.001933,0.004266,0.010179,0.005569,-0.00339,0.011275,0.003771,0.006128,0.016596,-0.000866,-0.001602,-0.012773,0.007784
Germany_Ilsenhöhle_LRJ_UP:RNI087__BC_45245__Cov_64 .28%,-0.091058,-0.07718,-0.085606,0.05491,0.002154,-0.005299,-0.002585,-0.005077,0.032315,-0.004009,0.015265,-0.010191,0.001784,-0.002615,-0.001357,-0.00769,0.007693,0.005828,-0.004399,0.01038,0.009982,0.002226,-0.004683,-0.017352,-0.000239
Germany_Ilsenhöhle_LRJ_UP:RNI012__BC_42355__Cov_50 .86%,-0.091058,-0.070072,-0.085606,0.057171,-0.000923,-0.011713,-0.001175,-0.003231,0.025565,-0.00893,0.011367,0.001499,0.000297,0.003028,0.0135 72,0.004641,-0.001434,0.011529,0.002891,0.010755,0.024207,-0.008903,-0.001725,-0.011327,0.011376
Germany_Ilsenhöhle_LRJ_UP:RNI004__BC_41660__Cov_71 .55%,-0.087644,-0.083273,-0.085229,0.057171,0.00277,-0.018128,-0.00564,0.000462,0.02986,-0.004738,0.004872,-3e-04,0.001487,0.001239,0.005022,-0.008088,-0.014473,0.006334,0.001634,0.004377,0.006489,0.010 14,-0.006162,-0.013857,0.00479
Germany_Ilsenhöhle_LRJ_UP:RNI013__BC_43040__Cov_99 .92%,-0.080814,-0.078196,-0.076933,0.061693,-0.003693,-0.016176,0.003055,-0.006461,0.015953,-0.006925,0.00747,-0.001349,-0.008474,-0.002477,0.004072,0.012066,0.011343,0.007221,0.009 553,0.002876,0.004866,0.008408,-0.008751,-0.020485,0.008981
Germany_Ilsenhöhle_LRJ_UP:RNI010__BC_43250__Cov_24 .64%,-0.060326,-0.030466,-0.055437,0.047804,0.004001,-0.006414,0.00188,-0.001615,0.024134,0.002369,0.008607,0,0.000595,-0.011836,0.009908,0.013524,-0.006128,-0.004561,0.002011,0.001376,-0.000125,-0.004822,-0.000246,-0.013978,-0.002754
Germany_Ilsenhöhle_LRJ_UP:RNI006__BC_41080__Cov_22 .64%,-0.045529,-0.022342,-0.043369,0.052003,0.015695,-0.002231,0.000705,-0.009,0.02352,-0.010205,0.009419,0.001199,0.011298,0,0.015744,0.0 0716,-0.003129,0.010008,0.001006,0.016008,-0.002371,0.004204,0.004437,-0.015544,0.002754
Germany_Ilsenhöhle_LRJ_UP:RNI081__BC_43016__Cov_46 .35%,-0.015935,-0.001016,-0.039975,0.047481,0.010156,-0.006693,-0.001645,0.000462,0.022907,0.000911,0.013803,-0.002248,-0.004162,0.006606,0.011129,0.000928,-0.009518,0.008742,0.003268,0.006003,0.012852,0.001 484,-0.001972,-0.001566,0.010059
Germany_Ilsenhöhle_LRJ_UP:RNI080__BC_42350__Cov_72 .57%,0.014797,0.030466,-0.027153,0.051034,0.011387,-0.003347,-0.00141,0.004384,0.015748,-0.009476,0.006496,0.002248,0.002081,0.004817,0.018 865,-0.003978,-0.012126,0.015963,0.005028,0.005628,0.009358,-0.00371,-0.002342,0.009037,0.005029

Upsilander
09-22-2024, 09:31 PM
Surprisingly not that African like for being 45 000y old. Some are lower but still less than Taforalt for example more than 35ky later. These OoA Homo Sapiens were clearly not Africans like it's often depicted but we can wonder what was the real Neanderthal influence on that early on. The ones with more Neanderthal though appear more African which is probably expected.


The two least African-like are the least Neanderthal admixed:

Distance to: Germany_Ilsenhöhle_LRJ_UP:RNI080__BC_42350__Cov_72 .57%
0.50306850 East_Asian
0.51211667 Amerindian_Native
0.65956280 African_Nigerian
0.70682772 Oceanian_Papuan


Distance to: Germany_Ilsenhöhle_LRJ_UP:RNI081__BC_43016__Cov_46 .35%
0.47699487 East_Asian
0.49591107 Amerindian_Native
0.63356086 African_Nigerian
0.68909972 Oceanian_Papuan


Distance to: Morocco_Taforalt:TAF012__BC_12505__Cov_20.16%
0.57131499 African_Nigerian
0.66953863 East_Asian
0.69048702 Amerindian_Native
0.82996742 Oceanian_Papuan


Not too far from Kostenki or BachoKiro but we see the ENA separation in East Eurasia:

Distance to: Russia_Kostenki14:Kostenki14__BC_36102__Cov_95.02%
0.49750576 East_Asian
0.49855865 Amerindian_Native
0.68097000 African_Nigerian
0.70539616 Oceanian_Papuan


Distance to: Bulgaria_BachoKiro_LatePleistocene:BK-1653_noUDG__BC_32998__Cov_87.41%
0.51999148 Amerindian_Native
0.52214531 East_Asian
0.68539408 African_Nigerian
0.71947345 Oceanian_Papuan


Distance to: China_Tianyuan:Tianyuan__BC_37615__Cov_76.92%
0.27506499 East_Asian
0.40158031 Amerindian_Native
0.61123540 Oceanian_Papuan
0.70820591 African_Nigerian

celticdragongod
09-22-2024, 10:28 PM
These samples are among those with the highest amount of Neanderthal DNA ever sequenced so far. Here, I have sorted them in descending order, from the most Neanderthal to the least.


Germany_Ilsenhöhle_LRJ_UP:RNI085__BC_42635__Cov_72 .88%,-0.093335,-0.06804,-0.089,0.049096,0.003693,-0.010598,-0.005405,-0.001615,0.029247,-0.007107,0.012666,-0.007493,-0.001933,0.004266,0.010179,0.005569,-0.00339,0.011275,0.003771,0.006128,0.016596,-0.000866,-0.001602,-0.012773,0.007784
Germany_Ilsenhöhle_LRJ_UP:RNI087__BC_45245__Cov_64 .28%,-0.091058,-0.07718,-0.085606,0.05491,0.002154,-0.005299,-0.002585,-0.005077,0.032315,-0.004009,0.015265,-0.010191,0.001784,-0.002615,-0.001357,-0.00769,0.007693,0.005828,-0.004399,0.01038,0.009982,0.002226,-0.004683,-0.017352,-0.000239
Germany_Ilsenhöhle_LRJ_UP:RNI012__BC_42355__Cov_50 .86%,-0.091058,-0.070072,-0.085606,0.057171,-0.000923,-0.011713,-0.001175,-0.003231,0.025565,-0.00893,0.011367,0.001499,0.000297,0.003028,0.0135 72,0.004641,-0.001434,0.011529,0.002891,0.010755,0.024207,-0.008903,-0.001725,-0.011327,0.011376
Germany_Ilsenhöhle_LRJ_UP:RNI004__BC_41660__Cov_71 .55%,-0.087644,-0.083273,-0.085229,0.057171,0.00277,-0.018128,-0.00564,0.000462,0.02986,-0.004738,0.004872,-3e-04,0.001487,0.001239,0.005022,-0.008088,-0.014473,0.006334,0.001634,0.004377,0.006489,0.010 14,-0.006162,-0.013857,0.00479
Germany_Ilsenhöhle_LRJ_UP:RNI013__BC_43040__Cov_99 .92%,-0.080814,-0.078196,-0.076933,0.061693,-0.003693,-0.016176,0.003055,-0.006461,0.015953,-0.006925,0.00747,-0.001349,-0.008474,-0.002477,0.004072,0.012066,0.011343,0.007221,0.009 553,0.002876,0.004866,0.008408,-0.008751,-0.020485,0.008981
Germany_Ilsenhöhle_LRJ_UP:RNI010__BC_43250__Cov_24 .64%,-0.060326,-0.030466,-0.055437,0.047804,0.004001,-0.006414,0.00188,-0.001615,0.024134,0.002369,0.008607,0,0.000595,-0.011836,0.009908,0.013524,-0.006128,-0.004561,0.002011,0.001376,-0.000125,-0.004822,-0.000246,-0.013978,-0.002754
Germany_Ilsenhöhle_LRJ_UP:RNI006__BC_41080__Cov_22 .64%,-0.045529,-0.022342,-0.043369,0.052003,0.015695,-0.002231,0.000705,-0.009,0.02352,-0.010205,0.009419,0.001199,0.011298,0,0.015744,0.0 0716,-0.003129,0.010008,0.001006,0.016008,-0.002371,0.004204,0.004437,-0.015544,0.002754
Germany_Ilsenhöhle_LRJ_UP:RNI081__BC_43016__Cov_46 .35%,-0.015935,-0.001016,-0.039975,0.047481,0.010156,-0.006693,-0.001645,0.000462,0.022907,0.000911,0.013803,-0.002248,-0.004162,0.006606,0.011129,0.000928,-0.009518,0.008742,0.003268,0.006003,0.012852,0.001 484,-0.001972,-0.001566,0.010059
Germany_Ilsenhöhle_LRJ_UP:RNI080__BC_42350__Cov_72 .57%,0.014797,0.030466,-0.027153,0.051034,0.011387,-0.003347,-0.00141,0.004384,0.015748,-0.009476,0.006496,0.002248,0.002081,0.004817,0.018 865,-0.003978,-0.012126,0.015963,0.005028,0.005628,0.009358,-0.00371,-0.002342,0.009037,0.005029

Distance to: CDG_scaled
0.18997822 Germany_Ilsenhöhle_LRJ_UP:RNI080__BC_42350__Cov_72 .57%
0.23511188 Germany_Ilsenhöhle_LRJ_UP:RNI081__BC_43016__Cov_46 .35%
0.26964809 Germany_Ilsenhöhle_LRJ_UP:RNI006__BC_41080__Cov_22 .64%
0.28904226 Germany_Ilsenhöhle_LRJ_UP:RNI010__BC_43250__Cov_24 .64%
0.34392667 Germany_Ilsenhöhle_LRJ_UP:RNI013__BC_43040__Cov_99 .92%
0.34788097 Germany_Ilsenhöhle_LRJ_UP:RNI012__BC_42355__Cov_50 .86%
0.34880201 Germany_Ilsenhöhle_LRJ_UP:RNI085__BC_42635__Cov_72 .88%
0.35277939 Germany_Ilsenhöhle_LRJ_UP:RNI087__BC_45245__Cov_64 .28%
0.35302839 Germany_Ilsenhöhle_LRJ_UP:RNI004__BC_41660__Cov_71 .55%

Target: CDG_scaled
Distance: 18.9978% / 0.18997822
100.0 Germany_Ilsenhöhle_LRJ_UP:RNI080__BC_42350__Cov_72 .57%

gixajo
09-22-2024, 10:33 PM
Surprisingly not that African like for being 45 000y old.

Just as curiosity, because distances are over 23: first closest modern "European" references are Tatar Crimea and Roma Balkans (and other Roma groups after this one).

They seem to have more affinity with Southern Asian modern populations.

It´s quite funny that they can be modeled getting better distances with the Davidski´s Ancient Standard model than modern Slavs.

https://i.imgur.com/pteUDLE.png

Upsilander
09-22-2024, 10:52 PM
Just as curiosity, because distances are over 23: first closest modern "European" references are Tatar Crimea and Roma Balkans (and other Roma groups after this one).

They seem to have more affinity with Southern Asian modern populations.

It´s quite funny that they can be modeled getting better distances with the Davidski´s Ancient Standard model than modern Slavs.

https://i.imgur.com/pteUDLE.png

I notice the least Neanderthal are also the least Jarawa and more Tepecik+Yamnaya which probably just signals something more related to what would become West Eurasian.