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The regional differences seem really huge in such an small country, some regions almost double others.
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Croatia is small in population but not in distance. It stretches quite far if you measure it. The Netherlands I think is the most diverse in terms of eye color. Up to 87% light eyed in Northern regions and only 54% Zeeland. That is a difference of more than 30% compared to Croatia's 25%. Portugal also has big differences. Up to 29% light in North and only 13% in some Southern provinces.
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I have recounted photos of a couple of schools which have higher quality images (about 5% of schools have much better quality photos than the rest) and so far all of them have around 3-5% more light eyes than their respected cities. This suggests that if photo quality was better in the entire set, the final numbers would be a few percent higher (there are medium/dark gray shades of eyes which take quite a HQ photo to distinguish from dark ones. Generally the better the image quality, the easier it is to distinguish mixed eyes from dark ones).
Hrvoje and Feiichi have accused me of dark washing Croatia with this study and I was 100% honest, however it's possible my final result of 37.6% for whole Croatia is a few percent lower than it should have been If I had the privilege of dealing with better images. TNH found 2.4% more light eyes than me (40%) and also Weisbach found 46% for entire Pannonian Croatia suggesting the total number is still above 40%.
Just thought it was my duty to report this finding in all fairness.
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